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教你快速了解制备型液相色谱

发布时间:2017/02/16 点击量:

江苏迪沃特仪器设备科技有限公司是一家专业从事从分析到制备分析的高新技术企业,目前公司提供一站式的实验室分析纯化服务。
新英体育 制备型加压液相色谱,按照色谱柱和样品量的大小,分为:(1)低压液相色谱;(2)中压液相色谱;(3)高压液相色谱;(4)快速色谱。低压、中压与高压液相色谱的压力范围之间会存在一定交叠,没有统一、明确的标准。

 

1
快速色谱
 

柱压通常为2bar(或30psi)左右,对于那些容易分离的简单混合物,由于快速色谱具有操作简便、经济等优点,常常是实验室的首选。但快速色谱不同于一般的层析分离,这种分离没有压力,而快速分离通常使用瓶装氮气加压,使流动相具有一定的流速,从而缩短了分离时间。Still等人率先于1978年详细研究了快速色谱,并于1981年获得了专利保护(美国专利4,293,422)。

快速色谱使用的柱子一般是玻璃柱,柱直径为3~10cm.长度为7~15cm。快速色谱中使用最广泛的固定相为硅胶。采用的粒径通常为:25~40μm,40~63μm或63~200μm的球形固定相。其它如键合相、氧化铝、聚酰胺吸附剂也常用作快速色谱的固定相使用。

2
低压色谱(LPLC)
 

新英体育 ZHUYAYIBANDIYU5bar(HUO75psi)。

新英体育 DIYASEPUYIBANSHIYOURUDONGBENG、JINYANGFAHEJIANCEQIZUCHENG,KEYILIANXUHUA,SHIXIANZIDONGDETIDULINXIHELIUFENSHOUJIDENGCAOZUO。SEPUZHUGUANYIBANSHIBOLIHUOJUHEWUCAILIAODE,ZHANGDUYIBANWEI240-440mm,NEIJINGWEI10-40mm。

新英体育 DUIYUDADUOSHUZAIZIWAIQUYOUXISHOUDEWUZHI,GUANGXUEJIANCEQIHENCHANGYONG。TIANLIAOYIBANSHIYONGRUANZHIDEPUJUTANG、QIONGZHITANG、XIANWEISU、HECHENGGAOJUWUHUOLIZIJIAOHUANJI,LIJINGYIBANWEI40-60μm。

3
中压液相色谱(MPLC)
 

ZHUYAZAI5-20bar(HUO75-300psi)ZHIJIAN,GUANGFANYONGYUSHIYANSHIHEGONGYEGUIMODESHENGWUZHIPIN(RUDONGWUZANGQITIQUYE、NONGSUOYE、TIYE、ZHIWUTIQUYE、SHENGWUJISHUFAJIAOYEDENG--WANGWANGXUYAOJINGGUOLVMOZUOCHUJIJINGHUA)DECHULI,YITIQUHUOCHUNHUASUOXUDECHANPIN。

ZHONGYAYEXIANGZHIBEISEPUDEZHUYAOBUJIANWEISHUYEBENG、JINYANGFA、JIANCEQI、LIUFENSHOUJIQIDENG,BIRURUISHIGONGSIDEZAOQIDEZHONGYAYEXIANGZHIBEISEPU,QISHUYEBENGZUIDALIUSUKEDA156mL/min,BINGPEIYOUZUNIQI,YIBAOZHENGYELIUDEWENDING;JINYANGQIPEIYOU0.5-50mLDEBUTONGTIJIDEDINGLIANGGUAN;JIANCEQIYOUZIWAIHESHICHAZHEGUANGJIANCEQI,LIUTONGCHITIJIBIJIAODA,YUNXUDALIULIANGLIUDONGXIANGTONGGUOERWUXUFENLIU;LIUFENSHOUJIQIYOUYUANPANSHIHEPAISHILIANGZHONG,YUANPANSHIDEJIESHOUGUANZUIDUODA80GE,ERHOUZHEZEGENGDUO;SEPUZHUNEIJING9-105mm,ZHANGDU250-1760mmBUDENG。

DUIYUYIBANZHONGYAZHIBEISEPU,DANGSEPUZHUZHIJINGJIAODASHI,ZHUTOUWANGWANGSHEJICHENGZHUIXINGHUOYOULEISIYUSANZHUANGDEYELIUDAOXIANGJIEGOU,SHIDEDANGDALIANGYANGPINJINRUDAOZHUTOUSHANGSHI,NENGXUNSUDIFENSANDAOZHENGGEZHUHENGJIEMIANSHANG,JISHIBEILIUDONGXIANGCHONGZOU,BIMIANLEYINYANGPINDEJUBUGUONONGERYINQIZHUCHAOFUHEHEPUDAIJIAKUAN。ZHUZITIANLIAOZECAIYONGBIJIAONAIYADEJIAOLIANGAIXINGDEDUOTANGNINGJIAO(RUSepharose CL,SuperoseDENG),JUHEWUWEIQIU,FUHECAILIAOJIEZHIHUOYINGZHISiO2JITIDEHUAXUEJIANHEXIANGDENG,LIJINGYIBANZAI25~40μm(ZUICHANGYONGDETIANLIAOCHICUNSHI15-25μm,25-40μmHUO40-63μm),KECAIYONGSHIFAHUOGANFAZHUANGZHU。

4
高压液相色谱(HPLC)
 

新英体育 SHIZHIZHUYAYIBANDAYU20bar(HUO300psi)DE“GAOYA(HUOGAOXIAO)YEXIANGSEPU”,TONGCHANGZHISUOYONGSEPUZHUDETABANSHUDAYU2000,YIBANSHIZAI2,000~20,000DEFANWEIZHIJIAN。

新英体育 DANGXUYAOCONGDALIANGDEWUZHIZHONGFENLICHUNHUABUZU1%DESUOXUCHENGFENSHI,FENLIGONGZUOJIANGHUISHIFENKUNNAN,WANGWANGZAICHUNHUADEZUIHOUJIEDUANXUYAOSHIYONG10μmHUOGENGXIAOKELIDEGAOXIAOTIANLIAO。WEIHUODESUOXUWEILIANGZUFEN,KECAIYONGRUXIAFENLISHOUDUAN:ZHIBEIXINGFENLI→BANZHIBEIXINGFENLI→FENXIXINGFENLI→CHANWU。WEITIGAOMEICIFENLIHUODECHUNPINDESHULIANG,ZHIBEIXINGGAOYAYEXIANGSEPUFENLITONGCHANGZAICHAOZAIQINGKUANGXIAYUNXING。

GAOYAYEXIANGSEPU,JIMUQIANCHANGYONGDEGAOXIAOYEXIANGSEPU。SEPUZHUNEITIANZHUANGDESHILIDUFANWEIJIAOZHAIDEWEIXIAOKELIGUDINGXIANG(3~30μm),WEISHILIUDONGXIANGLIUCHU,XUCAIYONGJIAOGAODEYALI,TONGSHIXITONGDEFUZAXINGJICHENGBENYIZENGDA,DANFENBIANLVKEDEDAOJIAODADETIGAO。ERTIANZHUANGJIAODAKELIDEGUDINGXIANGSHI,RUZHONGYAYEXIANGSEPUXITONG,ZHUANGZHUJIAORONGYI,ZHUDETONGTOUXINGJIAOGAO(ZHIXUJIAODIDEBENGYALI),KECAIYONGGENGDADESEPUZHUHEGENGJINGJIDEYIQI,YOUCIFENBIANLVYEJIAODI。

5
用分析型高压液相色谱进行制备型分离
 

DANGSUOXUCHUNHUAHEWUDELIANGHENSHAOSHI(WEIKEJIZHIJIHAOKE),KEYONGFENXIXINGSEPUZHUJINXINGDUOCIFENLI。XIAOGUOHELIYONGDAZHIJINGSEPUZHUJINXINGYICIXINGFENLIXIANGTONG。CAIYONGXIAOZHIJINGSEPUZHUSHI,KELIYONGYIYOUDEFENXIXINGYIQI,ERWUXUZAISEPUZHU、TIANLIAOJIFUJIANFANGMIANTOURUGENGDAZIJIN;LINGWAI,HAIKEZAIHENDACHENGDUSHANGBIMIANYOUYUFANGDASUOCHANSHENGDEWENTI,SHIFENLISUDUJIAKUAI。

XIAOZHIJINGSEPUZHUDECHICUNYIBANWEI250×4.6mm,TONGCHANGZHUANGYOUFANXIANGTIANLIAO,MEICIKEJINYANG5~100ug,TONGGUODUOCIJINYANGFENLI,KEHUODEZUGOUDECHUNPIN。LIRU,SuzukiDENG(1994)BAODAOCONGDOUKEZHIWUYUSHANDOU(Lupinus Hirsutus)ZHONGFENLIYIYUSHANDOUSHENGWUJIANTANGGANSHI,QIZUIHOUDEFENLIBUZHOUCAIYONGLiChrosorb Si60,5μm,250×4.6mmSEPUZHUJINXINGGAOYAYEXIANGSEPUFENLI,XITUOJIWEIHAN25%JIACHUNDEyiMIRONGYE-5%ANSHUI50:1。

新英体育 JINGCHANGXUYONGFENXIXINGSEPUZHUJINXINGFENLIDEYIGELINGYUSHIDUITAILEIHUAHEWUDECHUNHUA。SHENGWUHUOXINGTAIDEHANLIANGTONGCHANGHENDI,YONGFENXIXINGGAOYAYEXIANGSEPUZUOWEIZUIHOUDECHUNHUASHOUDUANSHI,BUHUISHISEPUZHUCHAOZAI。

WEILETIGAOFENLIXIAOLV,KEJIANGFENXIXINGGAOYAYEXIANGSEPUZHULIANJIEQILAISHIYONG。CISHIKECAIYONGKELIDUZAI20~30μmDETIANLIAO,YIBAOCHISHIDANGDETONGTOUXING,YOUQISHIDANGSHIYONGHANSHUIRONGJISHI。DANGSHIYONGJIWANDENGYOUJIRONGJISHI,YOUYULIUDONGXIANGDEZHANDUJIAODI,KESHIYONGKELIDUWEI10μmDETIANLIAO。

RANERYOUYUFENXIXINGSEPUXITONGWUFATIGONGDAGUIMOZHIBEIXINGFENLISUOXUDELIUSU,QIYINGYONGSHOUDAOYIDINGXIANZHI。

(LAIYUAN:HULIANWANG)

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